Journal: Journal of Animal Science and Biotechnology

Article Title: ZBED6 deletion activates FNDC1 -mediated PI3K/AKT and MAPK/ERK1/2 signaling pathways to promote myogenesis in pigs

doi: 10.1186/s40104-026-01504-w

Figure Lengend Snippet: ZBED6 deletion activates FNDC1 -mediated PI3K/AKT and MAPK/ERK1/2 signaling pathways. A RNA-seq data from ZBED6-deficient pigs, ZBED6 knockout myoblasts and myotubes, and FNDC1 knockdown C2C12 cells were comprehensively analyzed to screen downstream signaling pathways. B Protein expression levels and statistical analysis of p-AKT, AKT, p-mTOR and mTOR in WT and ZBED6 −/− pigs, n = 3 pigs/genotype. C and D Protein expression levels and statistical analysis, n = 3 pigs/genotype. E and F Protein expression levels and statistical analysis of p-mTOR, mTOR, p-AKT, AKT, p-ERK1/2 and ERK1/2, n = 3 independent cell-culture experiments. Data are expressed as mean ± SEM. In (B), and (D), the P values were determined by two-tailed Student's t -tests. In (F), the P values were determined by two-way ANOVA followed by Sidak's multiple comparisons test, different letters (a, b and c) between bars indicate P < 0.05

Article Snippet: Primary porcine skeletal muscle satellite cells were treated with 10 μmol/L LY294002, a PI3K inhibitor (MCE, HY-10108), or 1 μmol/L SCH772984, an ERK1/2 inhibitor (MCE, HY-50846).

Techniques: Protein-Protein interactions, RNA Sequencing, Knock-Out, Knockdown, Expressing, Analysis, Cell Culture, Two Tailed Test

Journal: Journal of Animal Science and Biotechnology

Article Title: ZBED6 deletion activates FNDC1 -mediated PI3K/AKT and MAPK/ERK1/2 signaling pathways to promote myogenesis in pigs

doi: 10.1186/s40104-026-01504-w

Figure Lengend Snippet: Inhibition of PI3K/AKT and MAPK/ERK1/2 pathways reduces the effects of the ZBED6-FNDC1 axis on myogenesis. A and B representative immunofluorescence staining for EdU ( A ) and percentage of EdU positive cells ( B ) in ZBED6 knockdown primary porcine skeletal muscle satellite cells treated with SCH772984, n = 3 independent cell-culture experiments. C and D . representative immunofluorescence staining for EdU ( C ) and percentage of EdU positive cells ( D ) in ZBED6 knockdown primary porcine skeletal muscle satellite cells treated with LY294002, n = 3 independent cell-culture experiments. E and F representative immunofluorescence staining for MYHC ( E ) and fusion index ( F ) in ZBED6 knockdown primary porcine skeletal muscle satellite cells treated with SCH772984, n = 3 independent cell-culture experiments. G and H representative immunofluorescence staining for MYHC ( G ) and fusion index ( H ) in ZBED6 knockdown primary porcine skeletal muscle satellite cells treated with LY294002, n = 3 independent cell-culture experiments. Data are represented as mean ± SEM. In (B), (D), (F), and (H), the P values were determined by two-way ANOVA followed by Sidak's multiple comparisons test, different letters (a and b) between bars indicate P < 0.05

Article Snippet: Primary porcine skeletal muscle satellite cells were treated with 10 μmol/L LY294002, a PI3K inhibitor (MCE, HY-10108), or 1 μmol/L SCH772984, an ERK1/2 inhibitor (MCE, HY-50846).

Techniques: Inhibition, Immunofluorescence, Staining, Knockdown, Cell Culture